Construct: shRNA TRCN0000303576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024831.6-1101s21c1
DNA Barcode:
GCAACATTATAGTCAACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TGS1 (96764)
Gene Description:
trimethylguanosine synthase 1
Transcript:
RefSeq NM_024831.6 (NON-CURRENT)
Match location:
Position 1101 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACATTATAGTCAACTTTA
Hairpin Sequence:
5'-CCGG-GCAACATTATAGTCAACTTTA-CTCGAG-TAAAGTTGACTATAATGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACATTATAGTCAACTTTACTCGAGTAAAGTTGACTATAATGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACATTATAGTCAACTTTACTCGAGTAAAGTTGACTATAATGTTGC-3'

Other clones with same target sequence:

(none)