Construct: shRNA TRCN0000303680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006306.2-2295s21c1
DNA Barcode:
CAACTTTGGGCCTCGCATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMC1A (8243)
Gene Description:
structural maintenance of chromosomes 1A
Transcript:
RefSeq NM_006306.2 (NON-CURRENT)
Match location:
Position 2295 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTTTGGGCCTCGCATTAA
Hairpin Sequence:
5'-CCGG-CAACTTTGGGCCTCGCATTAA-CTCGAG-TTAATGCGAGGCCCAAAGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTTTGGGCCTCGCATTAACTCGAGTTAATGCGAGGCCCAAAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTTTGGGCCTCGCATTAACTCGAGTTAATGCGAGGCCCAAAGTTG-3'

Other clones with same target sequence:

(none)