Construct: shRNA TRCN0000303729
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_020162.2-1252s21c1
- DNA Barcode:
- TCTACACGGAGGACGAGTTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DHX33 (56919)
- Gene Description:
- DEAH-box helicase 33
- Transcript:
- RefSeq NM_020162.2 (NON-CURRENT)
- Match location:
- Position 1252 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000303729)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCTACACGGAGGACGAGTTTG
- Hairpin Sequence:
-
5'-CCGG-TCTACACGGAGGACGAGTTTG-CTCGAG-CAAACTCGTCCTCCGTGTAGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCTACACGGAGGACGAGTTTGCTCGAGCAAACTCGTCCTCCGTGTAGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCTACACGGAGGACGAGTTTGCTCGAGCAAACTCGTCCTCCGTGTAGA-3'
Other clones with same target sequence:
(none)
