Construct: shRNA TRCN0000303764

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022463.3-1409s21c1
DNA Barcode:
CGGCCTCCTGAGACGTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NXN (64359)
Gene Description:
nucleoredoxin
Transcript:
RefSeq NM_022463.3 (NON-CURRENT)
Match location:
Position 1409 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303764)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCTCCTGAGACGTTATTT
Hairpin Sequence:
5'-CCGG-CGGCCTCCTGAGACGTTATTT-CTCGAG-AAATAACGTCTCAGGAGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCTCCTGAGACGTTATTTCTCGAGAAATAACGTCTCAGGAGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCTCCTGAGACGTTATTTCTCGAGAAATAACGTCTCAGGAGGCCG-3'

Other clones with same target sequence:

(none)