Construct: shRNA TRCN0000303802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004490.2-2168s21c1
DNA Barcode:
AGCCAGAAGTGACTTATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRB14 (2888)
Gene Description:
growth factor receptor bound protein 14
Transcript:
RefSeq NM_004490.2 (NON-CURRENT)
Match location:
Position 2168 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCAGAAGTGACTTATTAAA
Hairpin Sequence:
5'-CCGG-AGCCAGAAGTGACTTATTAAA-CTCGAG-TTTAATAAGTCACTTCTGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCAGAAGTGACTTATTAAACTCGAGTTTAATAAGTCACTTCTGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCAGAAGTGACTTATTAAACTCGAGTTTAATAAGTCACTTCTGGCT-3'

Other clones with same target sequence:

(none)