Construct: shRNA TRCN0000303834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014966.3-2245s21c1
DNA Barcode:
AGCGCTTCTCCCGATACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHX30 (22907)
Gene Description:
DExH-box helicase 30
Transcript:
RefSeq NM_014966.3 (CURRENT)
Match location:
Position 2245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGCTTCTCCCGATACTTTG
Hairpin Sequence:
5'-CCGG-AGCGCTTCTCCCGATACTTTG-CTCGAG-CAAAGTATCGGGAGAAGCGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGCTTCTCCCGATACTTTGCTCGAGCAAAGTATCGGGAGAAGCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGCTTCTCCCGATACTTTGCTCGAGCAAAGTATCGGGAGAAGCGCT-3'

Other clones with same target sequence:

(none)