Construct: shRNA TRCN0000303841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020831.3-891s21c1
DNA Barcode:
TTGTGGGCCAGGTGAACTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRTFA (57591)
Gene Description:
myocardin related transcription factor A
Transcript:
RefSeq NM_020831.3 (NON-CURRENT)
Match location:
Position 891 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTGGGCCAGGTGAACTATC
Hairpin Sequence:
5'-CCGG-TTGTGGGCCAGGTGAACTATC-CTCGAG-GATAGTTCACCTGGCCCACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTGGGCCAGGTGAACTATCCTCGAGGATAGTTCACCTGGCCCACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTGGGCCAGGTGAACTATCCTCGAGGATAGTTCACCTGGCCCACAA-3'

Other clones with same target sequence:

(none)