Construct: shRNA TRCN0000304377
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_022410.2-6220s21c1
- DNA Barcode:
- TACCCTTTGAGAATCTGATAC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Myh9 (17886)
- Gene Description:
- myosin, heavy polypeptide 9, non-muscle
- Transcript:
- RefSeq NM_022410.2 (NON-CURRENT)
- Match location:
- Position 6220 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000304377)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TACCCTTTGAGAATCTGATAC
- Hairpin Sequence:
-
5'-CCGG-TACCCTTTGAGAATCTGATAC-CTCGAG-GTATCAGATTCTCAAAGGGTA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTACCCTTTGAGAATCTGATACCTCGAGGTATCAGATTCTCAAAGGGTATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATACCCTTTGAGAATCTGATACCTCGAGGTATCAGATTCTCAAAGGGTA-3'
Other clones with same target sequence:
(none)
