Construct: shRNA TRCN0000304510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138679.5-688s21c1
DNA Barcode:
CAGTCGAGATCCTGATATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ash1l (192195)
Gene Description:
ash1 (absent, small, or homeotic)-like (Drosophila)
Transcript:
RefSeq NM_138679.5 (CURRENT)
Match location:
Position 688 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTCGAGATCCTGATATAAA
Hairpin Sequence:
5'-CCGG-CAGTCGAGATCCTGATATAAA-CTCGAG-TTTATATCAGGATCTCGACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTCGAGATCCTGATATAAACTCGAGTTTATATCAGGATCTCGACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTCGAGATCCTGATATAAACTCGAGTTTATATCAGGATCTCGACTG-3'

Other clones with same target sequence:

(none)