Construct: shRNA TRCN0000304552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023322.2-1423s21c1
DNA Barcode:
GCCAGTGAGAAGTACTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zkscan14 (67235)
Gene Description:
zinc finger with KRAB and SCAN domains 14
Transcript:
RefSeq NM_023322.2 (CURRENT)
Match location:
Position 1423 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTGAGAAGTACTATAAA
Hairpin Sequence:
5'-CCGG-GCCAGTGAGAAGTACTATAAA-CTCGAG-TTTATAGTACTTCTCACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTGAGAAGTACTATAAACTCGAGTTTATAGTACTTCTCACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTGAGAAGTACTATAAACTCGAGTTTATAGTACTTCTCACTGGC-3'

Other clones with same target sequence:

(none)