Construct: shRNA TRCN0000304683
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_009652.2-1575s21c1
- DNA Barcode:
- TCTGAGACTGACACCAGGTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Akt1 (11651)
- Gene Description:
- thymoma viral proto-oncogene 1
- Transcript:
- RefSeq NM_009652.2 (NON-CURRENT)
- Match location:
- Position 1575 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000304683)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCTGAGACTGACACCAGGTAT
- Hairpin Sequence:
-
5'-CCGG-TCTGAGACTGACACCAGGTAT-CTCGAG-ATACCTGGTGTCAGTCTCAGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCTGAGACTGACACCAGGTATCTCGAGATACCTGGTGTCAGTCTCAGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCTGAGACTGACACCAGGTATCTCGAGATACCTGGTGTCAGTCTCAGA-3'
Other clones with same target sequence:
-
TRCN0000022937
