Construct: shRNA TRCN0000304740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001077495.1-2984s21c1
DNA Barcode:
CCAGACGTTTCTAACCTTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3r1 (18708)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 1
Transcript:
RefSeq NM_001077495.1 (NON-CURRENT)
Match location:
Position 2984 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACGTTTCTAACCTTATC
Hairpin Sequence:
5'-CCGG-CCAGACGTTTCTAACCTTATC-CTCGAG-GATAAGGTTAGAAACGTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACGTTTCTAACCTTATCCTCGAGGATAAGGTTAGAAACGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACGTTTCTAACCTTATCCTCGAGGATAAGGTTAGAAACGTCTGG-3'

Other clones with same target sequence:

(none)