Construct: shRNA TRCN0000304940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201600.2-5857s21c1
DNA Barcode:
GCAGCTCCAGTCGCCATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo5b (17919)
Gene Description:
myosin VB
Transcript:
RefSeq NM_201600.2 (CURRENT)
Match location:
Position 5857 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTCCAGTCGCCATATAA
Hairpin Sequence:
5'-CCGG-GCAGCTCCAGTCGCCATATAA-CTCGAG-TTATATGGCGACTGGAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTCCAGTCGCCATATAACTCGAGTTATATGGCGACTGGAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTCCAGTCGCCATATAACTCGAGTTATATGGCGACTGGAGCTGC-3'

Other clones with same target sequence:

(none)