Construct: shRNA TRCN0000304989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016705.3-5073s21c1
DNA Barcode:
CTCAATCCATGGCTAGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif21a (16564)
Gene Description:
kinesin family member 21A
Transcript:
RefSeq NM_016705.3 (NON-CURRENT)
Match location:
Position 5073 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAATCCATGGCTAGATAAT
Hairpin Sequence:
5'-CCGG-CTCAATCCATGGCTAGATAAT-CTCGAG-ATTATCTAGCCATGGATTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAATCCATGGCTAGATAATCTCGAGATTATCTAGCCATGGATTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAATCCATGGCTAGATAATCTCGAGATTATCTAGCCATGGATTGAG-3'

Other clones with same target sequence:

(none)