Construct: shRNA TRCN0000305028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011375.2-597s21c1
DNA Barcode:
TGTGGACCCTGACCGGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St3gal5 (20454)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Transcript:
RefSeq NM_011375.2 (NON-CURRENT)
Match location:
Position 597 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGACCCTGACCGGATAAA
Hairpin Sequence:
5'-CCGG-TGTGGACCCTGACCGGATAAA-CTCGAG-TTTATCCGGTCAGGGTCCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGACCCTGACCGGATAAACTCGAGTTTATCCGGTCAGGGTCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGACCCTGACCGGATAAACTCGAGTTTATCCGGTCAGGGTCCACA-3'

Other clones with same target sequence:

(none)