Construct: shRNA TRCN0000305060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010145.2-1483s21c1
DNA Barcode:
TTCCTTCCCAGTCATACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ephx1 (13849)
Gene Description:
epoxide hydrolase 1, microsomal
Transcript:
RefSeq NM_010145.2 (NON-CURRENT)
Match location:
Position 1483 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCTTCCCAGTCATACTTAT
Hairpin Sequence:
5'-CCGG-TTCCTTCCCAGTCATACTTAT-CTCGAG-ATAAGTATGACTGGGAAGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCTTCCCAGTCATACTTATCTCGAGATAAGTATGACTGGGAAGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCTTCCCAGTCATACTTATCTCGAGATAAGTATGACTGGGAAGGAA-3'

Other clones with same target sequence:

(none)