Construct: shRNA TRCN0000305091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024213.2-1964s21c1
DNA Barcode:
GTAATGGACTAATTGGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Anapc4 (52206)
Gene Description:
anaphase promoting complex subunit 4
Transcript:
RefSeq NM_024213.2 (CURRENT)
Match location:
Position 1964 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAATGGACTAATTGGTATTA
Hairpin Sequence:
5'-CCGG-GTAATGGACTAATTGGTATTA-CTCGAG-TAATACCAATTAGTCCATTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAATGGACTAATTGGTATTACTCGAGTAATACCAATTAGTCCATTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAATGGACTAATTGGTATTACTCGAGTAATACCAATTAGTCCATTAC-3'

Other clones with same target sequence:

(none)