Construct: shRNA TRCN0000305173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173402.1-829s21c1
DNA Barcode:
TTGGACTAGATGCGAGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rgs12 (71729)
Gene Description:
regulator of G-protein signaling 12
Transcript:
RefSeq NM_173402.1 (NON-CURRENT)
Match location:
Position 829 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGACTAGATGCGAGTATTT
Hairpin Sequence:
5'-CCGG-TTGGACTAGATGCGAGTATTT-CTCGAG-AAATACTCGCATCTAGTCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGACTAGATGCGAGTATTTCTCGAGAAATACTCGCATCTAGTCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGACTAGATGCGAGTATTTCTCGAGAAATACTCGCATCTAGTCCAA-3'

Other clones with same target sequence:

(none)