Construct: shRNA TRCN0000305252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011883.3-799s21c1
DNA Barcode:
CTTCGTAAAGATCAACTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf13 (24017)
Gene Description:
ring finger protein 13
Transcript:
RefSeq NM_011883.3 (NON-CURRENT)
Match location:
Position 799 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGTAAAGATCAACTTAAA
Hairpin Sequence:
5'-CCGG-CTTCGTAAAGATCAACTTAAA-CTCGAG-TTTAAGTTGATCTTTACGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGTAAAGATCAACTTAAACTCGAGTTTAAGTTGATCTTTACGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGTAAAGATCAACTTAAACTCGAGTTTAAGTTGATCTTTACGAAG-3'

Other clones with same target sequence:

(none)