Construct: shRNA TRCN0000305295

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007623.2-1317s21c1
DNA Barcode:
GTCAAGCGGGACACCGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbx2 (12416)
Gene Description:
chromobox 2
Transcript:
RefSeq NM_007623.2 (NON-CURRENT)
Match location:
Position 1317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305295)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAAGCGGGACACCGTTAAA
Hairpin Sequence:
5'-CCGG-GTCAAGCGGGACACCGTTAAA-CTCGAG-TTTAACGGTGTCCCGCTTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAAGCGGGACACCGTTAAACTCGAGTTTAACGGTGTCCCGCTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAAGCGGGACACCGTTAAACTCGAGTTTAACGGTGTCCCGCTTGAC-3'

Other clones with same target sequence:

(none)