Construct: shRNA TRCN0000305295
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_007623.2-1317s21c1
- DNA Barcode:
- GTCAAGCGGGACACCGTTAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cbx2 (12416)
- Gene Description:
- chromobox 2
- Transcript:
- RefSeq NM_007623.2 (NON-CURRENT)
- Match location:
- Position 1317 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000305295)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTCAAGCGGGACACCGTTAAA
- Hairpin Sequence:
-
5'-CCGG-GTCAAGCGGGACACCGTTAAA-CTCGAG-TTTAACGGTGTCCCGCTTGAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTCAAGCGGGACACCGTTAAACTCGAGTTTAACGGTGTCCCGCTTGACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTCAAGCGGGACACCGTTAAACTCGAGTTTAACGGTGTCCCGCTTGAC-3'
Other clones with same target sequence:
(none)
