Construct: shRNA TRCN0000305344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028173.4-1271s21c1
DNA Barcode:
ATCGGAAGCTGACTGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tram1 (72265)
Gene Description:
translocating chain-associating membrane protein 1
Transcript:
RefSeq NM_028173.4 (NON-CURRENT)
Match location:
Position 1271 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGGAAGCTGACTGATTATT
Hairpin Sequence:
5'-CCGG-ATCGGAAGCTGACTGATTATT-CTCGAG-AATAATCAGTCAGCTTCCGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGGAAGCTGACTGATTATTCTCGAGAATAATCAGTCAGCTTCCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGGAAGCTGACTGATTATTCTCGAGAATAATCAGTCAGCTTCCGAT-3'

Other clones with same target sequence:

(none)