Construct: shRNA TRCN0000305346

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024225.4-1485s21c1
DNA Barcode:
TGTTACTTGGACATAACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx5 (69178)
Gene Description:
sorting nexin 5
Transcript:
RefSeq NM_024225.4 (NON-CURRENT)
Match location:
Position 1485 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305346)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTACTTGGACATAACTAAT
Hairpin Sequence:
5'-CCGG-TGTTACTTGGACATAACTAAT-CTCGAG-ATTAGTTATGTCCAAGTAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTACTTGGACATAACTAATCTCGAGATTAGTTATGTCCAAGTAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTACTTGGACATAACTAATCTCGAGATTAGTTATGTCCAAGTAACA-3'

Other clones with same target sequence:

(none)