Construct: shRNA TRCN0000305376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081223.1-555s21c1
DNA Barcode:
ATCCGACAGCAGAACCTTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbbp8 (225182)
Gene Description:
retinoblastoma binding protein 8, endonuclease
Transcript:
RefSeq NM_001081223.1 (NON-CURRENT)
Match location:
Position 555 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCGACAGCAGAACCTTAAG
Hairpin Sequence:
5'-CCGG-ATCCGACAGCAGAACCTTAAG-CTCGAG-CTTAAGGTTCTGCTGTCGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCGACAGCAGAACCTTAAGCTCGAGCTTAAGGTTCTGCTGTCGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCGACAGCAGAACCTTAAGCTCGAGCTTAAGGTTCTGCTGTCGGAT-3'

Other clones with same target sequence:

(none)