Construct: shRNA TRCN0000305462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145420.1-457s21c1
DNA Barcode:
CGATCCCTTAGTACCAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2d1 (216080)
Gene Description:
ubiquitin-conjugating enzyme E2D 1
Transcript:
RefSeq NM_145420.1 (NON-CURRENT)
Match location:
Position 457 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCCCTTAGTACCAGATAT
Hairpin Sequence:
5'-CCGG-CGATCCCTTAGTACCAGATAT-CTCGAG-ATATCTGGTACTAAGGGATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCCCTTAGTACCAGATATCTCGAGATATCTGGTACTAAGGGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCCCTTAGTACCAGATATCTCGAGATATCTGGTACTAAGGGATCG-3'

Other clones with same target sequence:

(none)