Construct: shRNA TRCN0000305467
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133941.2-2733s21c1
- DNA Barcode:
- GACATGAACACTTCAAGTATG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Dhx32 (101437)
- Gene Description:
- DEAH (Asp-Glu-Ala-His) box polypeptide 32
- Transcript:
- RefSeq NM_133941.2 (CURRENT)
- Match location:
- Position 2733 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000305467)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GACATGAACACTTCAAGTATG
- Hairpin Sequence:
-
5'-CCGG-GACATGAACACTTCAAGTATG-CTCGAG-CATACTTGAAGTGTTCATGTC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGACATGAACACTTCAAGTATGCTCGAGCATACTTGAAGTGTTCATGTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGACATGAACACTTCAAGTATGCTCGAGCATACTTGAAGTGTTCATGTC-3'
Other clones with same target sequence:
(none)
