Construct: shRNA TRCN0000305484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009186.4-585s21c1
DNA Barcode:
TGCTGATGTGTCTATTGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tra2b (20462)
Gene Description:
transformer 2 beta homolog (Drosophila)
Transcript:
RefSeq NM_009186.4 (NON-CURRENT)
Match location:
Position 585 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGATGTGTCTATTGTATA
Hairpin Sequence:
5'-CCGG-TGCTGATGTGTCTATTGTATA-CTCGAG-TATACAATAGACACATCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGATGTGTCTATTGTATACTCGAGTATACAATAGACACATCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGATGTGTCTATTGTATACTCGAGTATACAATAGACACATCAGCA-3'

Other clones with same target sequence:

(none)