Construct: shRNA TRCN0000305487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018829.3-358s21c1
DNA Barcode:
GGATAATGTGGTCATAGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap3m1 (55946)
Gene Description:
adaptor-related protein complex 3, mu 1 subunit
Transcript:
RefSeq NM_018829.3 (NON-CURRENT)
Match location:
Position 358 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATAATGTGGTCATAGTATA
Hairpin Sequence:
5'-CCGG-GGATAATGTGGTCATAGTATA-CTCGAG-TATACTATGACCACATTATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATAATGTGGTCATAGTATACTCGAGTATACTATGACCACATTATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATAATGTGGTCATAGTATACTCGAGTATACTATGACCACATTATCC-3'

Other clones with same target sequence:

(none)