Construct: shRNA TRCN0000305493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019490.1-3056s21c1
DNA Barcode:
ATGATAGAGATTACGTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uso1 (56041)
Gene Description:
USO1 vesicle docking factor
Transcript:
RefSeq NM_019490.1 (CURRENT)
Match location:
Position 3056 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGATAGAGATTACGTCATTA
Hairpin Sequence:
5'-CCGG-ATGATAGAGATTACGTCATTA-CTCGAG-TAATGACGTAATCTCTATCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGATAGAGATTACGTCATTACTCGAGTAATGACGTAATCTCTATCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGATAGAGATTACGTCATTACTCGAGTAATGACGTAATCTCTATCAT-3'

Other clones with same target sequence:

(none)