Construct: shRNA TRCN0000305550

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015786.1-654s21c1
DNA Barcode:
CAAGAAGGTTGCAGCCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h1c (50708)
Gene Description:
histone cluster 1, H1c
Transcript:
RefSeq NM_015786.1 (NON-CURRENT)
Match location:
Position 654 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305550)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAAGGTTGCAGCCAAGAA
Hairpin Sequence:
5'-CCGG-CAAGAAGGTTGCAGCCAAGAA-CTCGAG-TTCTTGGCTGCAACCTTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAAGGTTGCAGCCAAGAACTCGAGTTCTTGGCTGCAACCTTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAAGGTTGCAGCCAAGAACTCGAGTTCTTGGCTGCAACCTTCTTG-3'

Other clones with same target sequence:

(none)