Construct: shRNA TRCN0000305600

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018877.2-840s21c1
DNA Barcode:
TGCTATCTGGGAACCATATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Setdb1 (84505)
Gene Description:
SET domain, bifurcated 1
Transcript:
RefSeq NM_018877.2 (NON-CURRENT)
Match location:
Position 840 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305600)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTATCTGGGAACCATATTG
Hairpin Sequence:
5'-CCGG-TGCTATCTGGGAACCATATTG-CTCGAG-CAATATGGTTCCCAGATAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTATCTGGGAACCATATTGCTCGAGCAATATGGTTCCCAGATAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTATCTGGGAACCATATTGCTCGAGCAATATGGTTCCCAGATAGCA-3'

Other clones with same target sequence:

(none)