Construct: shRNA TRCN0000305727

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013651.4-251s21c1
DNA Barcode:
AGAACCACTTGGGATCCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sf3a2 (20222)
Gene Description:
splicing factor 3a, subunit 2
Transcript:
RefSeq NM_013651.4 (CURRENT)
Match location:
Position 251 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305727)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACCACTTGGGATCCTATG
Hairpin Sequence:
5'-CCGG-AGAACCACTTGGGATCCTATG-CTCGAG-CATAGGATCCCAAGTGGTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACCACTTGGGATCCTATGCTCGAGCATAGGATCCCAAGTGGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACCACTTGGGATCCTATGCTCGAGCATAGGATCCCAAGTGGTTCT-3'

Other clones with same target sequence:

(none)