Construct: shRNA TRCN0000305762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145390.4-2919s21c1
DNA Barcode:
AGGAGTCAGTGGGACCTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnpo2 (212999)
Gene Description:
transportin 2 (importin 3, karyopherin beta 2b)
Transcript:
RefSeq NM_145390.4 (CURRENT)
Match location:
Position 2919 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGTCAGTGGGACCTAAAT
Hairpin Sequence:
5'-CCGG-AGGAGTCAGTGGGACCTAAAT-CTCGAG-ATTTAGGTCCCACTGACTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGTCAGTGGGACCTAAATCTCGAGATTTAGGTCCCACTGACTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGTCAGTGGGACCTAAATCTCGAGATTTAGGTCCCACTGACTCCT-3'

Other clones with same target sequence:

(none)