Construct: shRNA TRCN0000305835

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146124.4-1914s21c1
DNA Barcode:
GCTTGGTTCTTCTAGGATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap1 (228359)
Gene Description:
Rho GTPase activating protein 1
Transcript:
RefSeq NM_146124.4 (CURRENT)
Match location:
Position 1914 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305835)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGGTTCTTCTAGGATTTA
Hairpin Sequence:
5'-CCGG-GCTTGGTTCTTCTAGGATTTA-CTCGAG-TAAATCCTAGAAGAACCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGGTTCTTCTAGGATTTACTCGAGTAAATCCTAGAAGAACCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGGTTCTTCTAGGATTTACTCGAGTAAATCCTAGAAGAACCAAGC-3'

Other clones with same target sequence:

(none)