Construct: shRNA TRCN0000305837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009035.4-309s21c1
DNA Barcode:
TAGGGAAGCTATGCGAAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbpj (19664)
Gene Description:
recombination signal binding protein for immunoglobulin kappa J region
Transcript:
RefSeq NM_009035.4 (NON-CURRENT)
Match location:
Position 309 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGGAAGCTATGCGAAATTA
Hairpin Sequence:
5'-CCGG-TAGGGAAGCTATGCGAAATTA-CTCGAG-TAATTTCGCATAGCTTCCCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGGAAGCTATGCGAAATTACTCGAGTAATTTCGCATAGCTTCCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGGAAGCTATGCGAAATTACTCGAGTAATTTCGCATAGCTTCCCTA-3'

Other clones with same target sequence:

(none)