Construct: shRNA TRCN0000305950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017462.2-1731s21c1
DNA Barcode:
TCTCAGGAGAGAGGTACAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Polg (18975)
Gene Description:
polymerase (DNA directed), gamma
Transcript:
RefSeq NM_017462.2 (CURRENT)
Match location:
Position 1731 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCAGGAGAGAGGTACAAAG
Hairpin Sequence:
5'-CCGG-TCTCAGGAGAGAGGTACAAAG-CTCGAG-CTTTGTACCTCTCTCCTGAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCAGGAGAGAGGTACAAAGCTCGAGCTTTGTACCTCTCTCCTGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCAGGAGAGAGGTACAAAGCTCGAGCTTTGTACCTCTCTCCTGAGA-3'

Other clones with same target sequence:

(none)