Construct: shRNA TRCN0000305973

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176839.1-392s21c1
DNA Barcode:
GTGCATTCTCATCTCCAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcc5 (27416)
Gene Description:
ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Transcript:
RefSeq NM_176839.1 (CURRENT)
Match location:
Position 392 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305973)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCATTCTCATCTCCAAATT
Hairpin Sequence:
5'-CCGG-GTGCATTCTCATCTCCAAATT-CTCGAG-AATTTGGAGATGAGAATGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCATTCTCATCTCCAAATTCTCGAGAATTTGGAGATGAGAATGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCATTCTCATCTCCAAATTCTCGAGAATTTGGAGATGAGAATGCAC-3'

Other clones with same target sequence:

(none)