Construct: shRNA TRCN0000305986

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009658.3-1193s21c1
DNA Barcode:
TACCAGTAGCCAAGGTGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akr1b3 (11677)
Gene Description:
aldo-keto reductase family 1, member B3 (aldose reductase)
Transcript:
RefSeq NM_009658.3 (CURRENT)
Match location:
Position 1193 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305986)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCAGTAGCCAAGGTGTTTC
Hairpin Sequence:
5'-CCGG-TACCAGTAGCCAAGGTGTTTC-CTCGAG-GAAACACCTTGGCTACTGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCAGTAGCCAAGGTGTTTCCTCGAGGAAACACCTTGGCTACTGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCAGTAGCCAAGGTGTTTCCTCGAGGAAACACCTTGGCTACTGGTA-3'

Other clones with same target sequence:

(none)