Construct: shRNA TRCN0000306035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008910.2-1475s21c1
DNA Barcode:
TTGTACAAAGTGCTCATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppm1a (19042)
Gene Description:
protein phosphatase 1A, magnesium dependent, alpha isoform
Transcript:
RefSeq NM_008910.2 (NON-CURRENT)
Match location:
Position 1475 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTACAAAGTGCTCATATTT
Hairpin Sequence:
5'-CCGG-TTGTACAAAGTGCTCATATTT-CTCGAG-AAATATGAGCACTTTGTACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTACAAAGTGCTCATATTTCTCGAGAAATATGAGCACTTTGTACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTACAAAGTGCTCATATTTCTCGAGAAATATGAGCACTTTGTACAA-3'

Other clones with same target sequence:

(none)