Construct: shRNA TRCN0000306144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021604.2-506s21c1
DNA Barcode:
CATGTCAGACCTGAATCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agrn (11603)
Gene Description:
agrin
Transcript:
RefSeq NM_021604.2 (NON-CURRENT)
Match location:
Position 506 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTCAGACCTGAATCATAT
Hairpin Sequence:
5'-CCGG-CATGTCAGACCTGAATCATAT-CTCGAG-ATATGATTCAGGTCTGACATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTCAGACCTGAATCATATCTCGAGATATGATTCAGGTCTGACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTCAGACCTGAATCATATCTCGAGATATGATTCAGGTCTGACATG-3'

Other clones with same target sequence:

(none)