Construct: shRNA TRCN0000306418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008014.3-1268s21c1
DNA Barcode:
CCATGGATGGACGAGTCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppm1g (14208)
Gene Description:
protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Transcript:
RefSeq NM_008014.3 (CURRENT)
Match location:
Position 1268 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGGATGGACGAGTCAATG
Hairpin Sequence:
5'-CCGG-CCATGGATGGACGAGTCAATG-CTCGAG-CATTGACTCGTCCATCCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGGATGGACGAGTCAATGCTCGAGCATTGACTCGTCCATCCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGGATGGACGAGTCAATGCTCGAGCATTGACTCGTCCATCCATGG-3'

Other clones with same target sequence:

(none)