Construct: shRNA TRCN0000306420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007715.5-2465s21c1
DNA Barcode:
CAGGACAGACAGATAAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clock (12753)
Gene Description:
circadian locomotor output cycles kaput
Transcript:
RefSeq NM_007715.5 (NON-CURRENT)
Match location:
Position 2465 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGACAGACAGATAAGATTT
Hairpin Sequence:
5'-CCGG-CAGGACAGACAGATAAGATTT-CTCGAG-AAATCTTATCTGTCTGTCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGACAGACAGATAAGATTTCTCGAGAAATCTTATCTGTCTGTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGACAGACAGATAAGATTTCTCGAGAAATCTTATCTGTCTGTCCTG-3'

Other clones with same target sequence:

(none)