Construct: shRNA TRCN0000306497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022417.3-195s21c1
DNA Barcode:
AGGCTGATAAAGCGGACAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itm2c (64294)
Gene Description:
integral membrane protein 2C
Transcript:
RefSeq NM_022417.3 (CURRENT)
Match location:
Position 195 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCTGATAAAGCGGACAAAG
Hairpin Sequence:
5'-CCGG-AGGCTGATAAAGCGGACAAAG-CTCGAG-CTTTGTCCGCTTTATCAGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCTGATAAAGCGGACAAAGCTCGAGCTTTGTCCGCTTTATCAGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCTGATAAAGCGGACAAAGCTCGAGCTTTGTCCGCTTTATCAGCCT-3'

Other clones with same target sequence:

(none)