Construct: shRNA TRCN0000306662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201357.1-1303s21c1
DNA Barcode:
ATCTGCAGCTACGAGGTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tssc1 (380752)
Gene Description:
tumor suppressing subtransferable candidate 1
Transcript:
RefSeq NM_201357.1 (NON-CURRENT)
Match location:
Position 1303 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTGCAGCTACGAGGTAATT
Hairpin Sequence:
5'-CCGG-ATCTGCAGCTACGAGGTAATT-CTCGAG-AATTACCTCGTAGCTGCAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTGCAGCTACGAGGTAATTCTCGAGAATTACCTCGTAGCTGCAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTGCAGCTACGAGGTAATTCTCGAGAATTACCTCGTAGCTGCAGAT-3'

Other clones with same target sequence:

(none)