Construct: shRNA TRCN0000306664

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005605.1-882s21c1
DNA Barcode:
GAAAGGTTGCAAGGTGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aebp2 (11569)
Gene Description:
AE binding protein 2
Transcript:
RefSeq NM_001005605.1 (NON-CURRENT)
Match location:
Position 882 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306664)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGGTTGCAAGGTGTATAA
Hairpin Sequence:
5'-CCGG-GAAAGGTTGCAAGGTGTATAA-CTCGAG-TTATACACCTTGCAACCTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGGTTGCAAGGTGTATAACTCGAGTTATACACCTTGCAACCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGGTTGCAAGGTGTATAACTCGAGTTATACACCTTGCAACCTTTC-3'

Other clones with same target sequence:

(none)