Construct: shRNA TRCN0000306850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006117.2-196s21c1
DNA Barcode:
CCAGGAAACGAAGTGAAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ECI2 (10455)
Gene Description:
enoyl-CoA delta isomerase 2
Transcript:
RefSeq NM_006117.2 (CURRENT)
Match location:
Position 196 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAAACGAAGTGAAGCTA
Hairpin Sequence:
5'-CCGG-CCAGGAAACGAAGTGAAGCTA-CTCGAG-TAGCTTCACTTCGTTTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAAACGAAGTGAAGCTACTCGAGTAGCTTCACTTCGTTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAAACGAAGTGAAGCTACTCGAGTAGCTTCACTTCGTTTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000049385