Construct: shRNA TRCN0000306853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005415.3-1173s21c1
DNA Barcode:
GCCCTTATCGTCTGGTTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC20A1 (6574)
Gene Description:
solute carrier family 20 member 1
Transcript:
RefSeq NM_005415.3 (NON-CURRENT)
Match location:
Position 1173 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTATCGTCTGGTTCTTT
Hairpin Sequence:
5'-CCGG-GCCCTTATCGTCTGGTTCTTT-CTCGAG-AAAGAACCAGACGATAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTATCGTCTGGTTCTTTCTCGAGAAAGAACCAGACGATAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTATCGTCTGGTTCTTTCTCGAGAAAGAACCAGACGATAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000043053