Construct: shRNA TRCN0000306872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005936.2-2630s21c1
DNA Barcode:
ATCGTGCAGGCAACGACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AFDN (4301)
Gene Description:
afadin, adherens junction formation factor
Transcript:
RefSeq NM_005936.2 (NON-CURRENT)
Match location:
Position 2630 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGTGCAGGCAACGACTTTG
Hairpin Sequence:
5'-CCGG-ATCGTGCAGGCAACGACTTTG-CTCGAG-CAAAGTCGTTGCCTGCACGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGTGCAGGCAACGACTTTGCTCGAGCAAAGTCGTTGCCTGCACGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGTGCAGGCAACGACTTTGCTCGAGCAAAGTCGTTGCCTGCACGAT-3'

Other clones with same target sequence:

(none)