Construct: shRNA TRCN0000306971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005375.2-1670s21c1
DNA Barcode:
CCCTCTCATCTAGTAGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYB (4602)
Gene Description:
MYB proto-oncogene, transcription factor
Transcript:
RefSeq NM_005375.2 (NON-CURRENT)
Match location:
Position 1670 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTCATCTAGTAGAAGAT
Hairpin Sequence:
5'-CCGG-CCCTCTCATCTAGTAGAAGAT-CTCGAG-ATCTTCTACTAGATGAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTCATCTAGTAGAAGATCTCGAGATCTTCTACTAGATGAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTCATCTAGTAGAAGATCTCGAGATCTTCTACTAGATGAGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000040061