Construct: shRNA TRCN0000307144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021079.3-706s21c1
DNA Barcode:
CCAGCAAACATCCATATCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NMT1 (4836)
Gene Description:
N-myristoyltransferase 1
Transcript:
RefSeq NM_021079.3 (NON-CURRENT)
Match location:
Position 706 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAAACATCCATATCTAT
Hairpin Sequence:
5'-CCGG-CCAGCAAACATCCATATCTAT-CTCGAG-ATAGATATGGATGTTTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAAACATCCATATCTATCTCGAGATAGATATGGATGTTTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAAACATCCATATCTATCTCGAGATAGATATGGATGTTTGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000035709