Construct: shRNA TRCN0000307207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021204.3-642s21c1
DNA Barcode:
GCAGAGTTCTTTGCAGATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ENOPH1 (58478)
Gene Description:
enolase-phosphatase 1
Transcript:
RefSeq NM_021204.3 (NON-CURRENT)
Match location:
Position 642 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTTCTTTGCAGATGTA
Hairpin Sequence:
5'-CCGG-GCAGAGTTCTTTGCAGATGTA-CTCGAG-TACATCTGCAAAGAACTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTTCTTTGCAGATGTACTCGAGTACATCTGCAAAGAACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTTCTTTGCAGATGTACTCGAGTACATCTGCAAAGAACTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000082749